human colon cancer caco 2 cell line  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Activity of the Antimicrobial Myristoylated Peptide Myr-B in HeLa Cells: Cytotoxic, Membrane-Disruptive and Proteomic Insights

doi: 10.3390/ijms27093918

Figure Lengend Snippet: The cytotoxic effect of Myr-B peptide against human colon cancer Caco-2 cells. Cell viability was assessed using the MTT assay for the Caco-2 cell line after 3 h and 24 h of incubation with N-myristoylated Myr-B peptide ( a ) and corresponding non-myristoylated Pep-B peptide ( b ). Data are expressed as a percentage of viable cells in the presence of different peptide concentrations (2.5–50 µM) compared to untreated Caco-2 cells (negative control). Positive control represents cells treated with 2% NaN 3 . Untreated cells serve as negative control. All experiments were performed using two independent replicates, each with at least three repeats. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s post hoc test (GraphPad Prism 11.0). Difference from the negative control was considered statistically significant as follows: ** adjusted p -value < 0.01; **** p -value < 0.0001.

Article Snippet: The human colon cancer Caco-2 cell line (ATCC HTB-37) was cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 1% ( w / v ) penicillin–streptomycin, 1% Non-Essential Amino Acids and 1% sodium pyruvate and maintained at 37 °C in a humidified incubator with 5% CO 2 [ ].

Techniques: MTT Assay, Incubation, Negative Control, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Activity of the Antimicrobial Myristoylated Peptide Myr-B in HeLa Cells: Cytotoxic, Membrane-Disruptive and Proteomic Insights

doi: 10.3390/ijms27093918

Figure Lengend Snippet: Evaluation of Myr-B peptide-induced death of human cervical cancer HeLa ( a ) and colon cancer Caco-2 ( b ) cells. Cell membrane integrity was assessed through lactate dehydrogenase (LDH) release from untreated HeLa or Caco-2 cells (negative control) after 3 h exposure of HeLa and Caco-2 cells to their respective IC 50 doses of Myr-B peptide (38 μM and 50 μM, respectively) and from HeLa or Caco-2 cells treated with a lysis solution to release all LDH (positive control). The exposure to Pep-B was carried out under the same conditions as the corresponding treatment with the Myr-B peptide for each cancer cell line. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s post hoc test (GraphPad Prism 11.0). Difference from the negative control was considered statistically significant as follows: * adjusted p -value < 0.05; *** p -value < 0.001; **** p -value < 0.0001.

Article Snippet: The human colon cancer Caco-2 cell line (ATCC HTB-37) was cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 1% ( w / v ) penicillin–streptomycin, 1% Non-Essential Amino Acids and 1% sodium pyruvate and maintained at 37 °C in a humidified incubator with 5% CO 2 [ ].

Techniques: Membrane, Negative Control, Lysis, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Activity of the Antimicrobial Myristoylated Peptide Myr-B in HeLa Cells: Cytotoxic, Membrane-Disruptive and Proteomic Insights

doi: 10.3390/ijms27093918

Figure Lengend Snippet: SEM analysis of Myr-B peptide-induced effects on human colon cancer Caco-2 cells. SEM micrographs (acquired and processed using JEOL inTouchScope Interface software) of untreated Caco-2 cells (negative control) ( a , b ), Caco-2 cells after 3 h exposure to 50 μM Myr-B ( c , d ) and 50 μM Pep-B ( e , f ) and Caco-2 cells treated with 2% NaN 3 (positive control) ( g , h ). Magnification and scale bars = ( a – d ) 1500× and 10 μm, respectively; ( e – h ) 10,000× and 1 μm, respectively.

Article Snippet: The human colon cancer Caco-2 cell line (ATCC HTB-37) was cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 1% ( w / v ) penicillin–streptomycin, 1% Non-Essential Amino Acids and 1% sodium pyruvate and maintained at 37 °C in a humidified incubator with 5% CO 2 [ ].

Techniques: Software, Negative Control, Positive Control

Journal: Iranian Journal of Pharmaceutical Research : IJPR

Article Title: Gamabufotalin Suppresses Colorectal Cancer Growth via Oxidative Stress-Induced Apoptosis and DNA Synthesis Inhibition

doi: 10.5812/ijpr-169859

Figure Lengend Snippet: In vitro experiments demonstrated that gamabufotalin (GA) has a dose-dependent inhibitory effect on colorectal cancer (CRC) cells. A, the chemical formula of GA; B, SW480, HCT-116, HT-29, NCM460, and 293T cells were treated with 0-20000 nM GA for 48 hours, and viability was initially determined using the CCK-8 kit. Each concentration point was repeated six times (n = 6). C, CRC SW480, HCT-116, and HT-29 cells were co-incubated with GA at concentrations ranging from 0 to 200 nM for 48 hours, and the relative cell viability of each experimental group was evaluated using the CCK-8 kit. D, the cell viability of SW480, HCT-116, HT-29, NCM460, and 293T cells was measured using the CCK-8 kit at a GA concentration of 50 nM. E, the cell viability of CRC SW480 and HCT-116 cells was measured under a serum concentration of 1% in the culture condition. F, SW480 cells were treated with GA for 48 hours, and the morphological changes in SW480 cells caused by GA were photographed using an inverted microscope. G, SW480 cells were treated with different concentrations of GA for 24 hours, and the width of the scratch was measured, and the cell scratch closure rate was calculated. Post hoc analysis using LSD was performed after one-way ANOVA to evaluate significance, ** P < 0.01.

Article Snippet: SW480, HCT-116, NCM460, and 293T human colon cancer cell lines were procured from the American Type Culture Collection (ATCC).

Techniques: In Vitro, CCK-8 Assay, Concentration Assay, Incubation, Inverted Microscopy

Journal: eLife

Article Title: Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

doi: 10.7554/eLife.107104

Figure Lengend Snippet: ( A ) Kaplan-Meier analysis of relapse-free survival in KRAS -mutant colorectal cancer patients with high or low expression levels of UBE2V1 and UBE2V2. ( B and E ) The knockdown efficiency assays. The relative mRNA levels were normalized to GAPDH . ( C–G ) Assays to evaluate the effects of UBE2V1- and UBE2V2-RNAi on colony formation and cell viability in SW480 and HCT116 cells. In ( B, C, E, and F ), three independent replicates were conducted, and statistical significance was determined using t test. In ( D and G ), five ( D ) and six ( G ) independent replicates were conducted at each time point, and statistical significance was determined using two-way ANOVA with multiple comparisons. * (p<0.05), *** (p<0.001), and **** (p<0.0001).

Article Snippet: The human colon cancer cell lines SW480 and HCT116, as well as the human embryonic kidney cell line 293T, were purchased from the American Type Culture Collection (ATCC), authenticated by STR profiling, and tested negative for mycoplasma contamination.

Techniques: Mutagenesis, Expressing, Knockdown

Journal: eLife

Article Title: Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

doi: 10.7554/eLife.107104

Figure Lengend Snippet: ( A and C ) The knockdown efficiency assays. The relative mRNA levels were normalized to GAPDH . Three independent replicates were conducted, and statistical significance was determined using one-way ANOVA. ( B and D ) Assays to evaluate the effects of UBE2V1- and UBE2V2-RNAi on colony formation and cell viability in SW480 cells. Six independent replicates were conducted at each time point, and statistical significance was determined using two-way ANOVA with multiple comparisons. n.s . (p>0.05), * (p<0.05), ** (p<0.01), *** (p<0.001), and **** (p<0.0001).

Article Snippet: The human colon cancer cell lines SW480 and HCT116, as well as the human embryonic kidney cell line 293T, were purchased from the American Type Culture Collection (ATCC), authenticated by STR profiling, and tested negative for mycoplasma contamination.

Techniques: Knockdown

Journal: eLife

Article Title: Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

doi: 10.7554/eLife.107104

Figure Lengend Snippet: ( A ) Western blotting to confirm the transient overexpression of UBE2V1 and UBE2V2 in SW480 and HCT116 cell lines. β-Actin was used as the loading control. ( B ) Western blotting to confirm the stable overexpression of UBE2V1 and UBE2V2 in SW480 cells, with UBE2V1-OE #1 and UBE2V2 #3 cell lines utilized in subcutaneous tumorigenesis assays. α-Tubulin was used as the loading control. In both ( A ) and ( B ), cells transfected with an empty overexpression vector served as the control. Figure 9—figure supplement 1—source data 1. PDF files that contain original western blots indicating the relevant bands and treatments. Figure 9—figure supplement 1—source data 2. Original files for western blot analysis.

Article Snippet: The human colon cancer cell lines SW480 and HCT116, as well as the human embryonic kidney cell line 293T, were purchased from the American Type Culture Collection (ATCC), authenticated by STR profiling, and tested negative for mycoplasma contamination.

Techniques: Western Blot, Over Expression, Control, Transfection, Plasmid Preparation

Journal: eLife

Article Title: Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

doi: 10.7554/eLife.107104

Figure Lengend Snippet: ( A and B ) 5-Ethynyl-2’-deoxyuridine (EdU) incorporation assays to assess the effects of UBE2V1 and UBE2V2 overexpression (OE) on cell proliferation in SW480 and HCT116 cells. Empty OE vector was used as the control. All images in ( A ) are of the same magnification. ( C–E ) Assays to evaluate the effects of UBE2V1- and UBE2V2-OE on colony formation and cell viability in SW480 and HCT116 cells. In ( B and D ), three independent replicates were conducted, and statistical significance was determined using one-way ANOVA. In ( E ), five independent replicates were conducted, and statistical significance was determined using two-way ANOVA with multiple comparisons. * (p<0.05), ** (p<0.01), and **** (p<0.0001).

Article Snippet: The human colon cancer cell lines SW480 and HCT116, as well as the human embryonic kidney cell line 293T, were purchased from the American Type Culture Collection (ATCC), authenticated by STR profiling, and tested negative for mycoplasma contamination.

Techniques: Over Expression, Plasmid Preparation, Control